Pacbio Long-Read Sequencing Services
At AGRF, we offer the PacBio® Revio system, a leading solution in Long-Read Sequencing. Revio enables researchers to explore the most complex regions of the genome with confidence. Featuring new SPRQ chemistry and competitive pricing, the Revio delivers exceptional read length and quality in different applications. Whether you’re studying structural variants, epigenetics, the microbiome or metagenomics, our advanced technology combined with our local expertise will support your research goals.
Long-Read Sequencing has provided novel findings in areas such as rare disease diagnosis, oncology, infectious disease studies, microbiota analysis, and clinical research through HLA-typing and pharmacogenomics analysis.
Our Accreditation
AGRF is recognised as a PacBio Certified Service Provider (CSP) and an ISO17025 accredited service for Long-Read Sequencing. Our Long-Read services operate in compliance with the ISO17025 international standard, ensuring sample traceability, integrity, and accuracy.
Quick Guide for Long-Read Sequencing: Drop down arrow to view
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Large insert capability: Sequencing of larger inserts (15-20 kb) enables the resolution of large variants and mapping of difficult regions of the genome
Comprehensive genomic coverage: Long-Read Sequencing excels at resolving repetitive regions, structural variants, and complex genomes that are challenging for short-read technologies
Enhanced accuracy: With 90% of bases Q30+ and median read accuracy ≥Q30, Revio delivers highly accurate and reliable data, hence minimises assembly gaps and mapping error
Improve agricultural practices: Valuable for plant and animal genomes, which are often larger, more repetitive, or polyploid
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Rare disease research: Pinpoint mutations and structural variants linked to rare and inherited diseases
Structural Variant Analysis: Identify large-scale genomic rearrangements and variations with precision
Epigenomics: Explore DNA modifications to understand gene regulation and epigenetic mechanisms
Transcriptomics: Characterise whole transcriptomes in a single SMRT Cell 25M and increase detection of long isoforms using Kinnex Iso-Seq (RNA Seq) for detailed gene expression studies
Microbial and metagenomics: Characterise diverse microbial communities and uncover hard-to-detect species
Plant and animal genomics: Achieve high-quality de novo assemblies, identification of key traits for breeding programs, and exploration of biodiversity for conservation efforts
Pacbio Application Can Improve agricultural practices: Valuable for plant and animal genomes, which are often larger, more repetitive, or polyploid
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Whole Genome Sequencing (WGS)
Human WGS
Kinnex Iso-Seq (RNA Seq)
Full length 16S and ITS
Metagenomics
Amplicons
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Save time and effort by leaving extraction to us. AGRF’s expert Extraction service handles a wide range of sample types, delivering high-quality nucleic acid to meet the exact requirements of your project. Contact our team today for a quote or to discuss your extraction needs.
AGRF provides high molecular weight DNA extraction services. Sample types include:
Fresh and frozen blood (EDTA tubes only)
Flash frozen tissues (not stored in ethanol)
*Frozen plants
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Through our genome assembly services, powered by Dovetail Genomics, we deliver assemblies of the highest quality. Whether your focus is on species conservation, evolutionary biology or enhancing agricultural efficiencies, assembling a reference genome is often the first step in building a genetic understanding of your species of interest.
Our services support a wide range of applications including breeding programs, population studies, cultivar verification, pest research and disease screening. Whatever your industry needs, we’re here to provide the genomic tools and expertise to help you.Assembly link: Powered by Dovetail Genomics’ Assembly Link, get rapid genome assembly from sample to sequencing ready libraries in a single day.
Omni-C: Complimenting your long-read contigs, we utilise Hi-C technology to generate chromosome-scale, haplotype-resolved genome assemblies for both diploid and polyploid species. While advances in long-read sequencing have been significant, these platforms alone are often insufficient for assembling full chromosomes, except in the smallest genomes. Hi-C bridges this gap, transforming draft assemblies into chromosome-level resolutions, providing the most complete and biologically relevant assemblies possible.
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Our bioinformatics services transform raw sequencing data into meaningful insights—saving you time and resources. We offer tailored solutions, including custom analysis to meet your specific research objectives, and expert consultation to guide your study design and data interpretation. From quality control to pathway analysis, our services empower you to confidently navigate complex data and drive meaningful discoveries.
Our Long-Read bioinformatics offers standard analyses such as 16S profiling, human variant calling, genome assembly, methylation studies, and transcriptomics through IsoSeq, as well as a wide range of custom bioinformatics solutions. Whether for routine or specialised projects, AGRF is committed to delivering reliable and actionable insights for your research needs.
Contact our specialist team today
Our team is always available to talk you through your next project or any questions you might have - get in touch today to arrange a consultation.