Your Partners in Genetic Identification

Human Cell Line ID

• AGRF’s Human Cell Line Identification service is a method useful in genotyping and is particularly useful in tissue culture strain identification.

• The American Tissue Culture Collection Standards Development Organization Workgroup issued standard ASN-0002, which recommends the use of at least eight STR loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317 and D5S818), plus Amelogenin for gender identification, for human cell line authentication.

• The International Cell Line Authentication Committee (ICLAC) recommends that human cell lines are tested using short tandem repeat (STR) profiling in accordance with the standard ANSI/ATCC ASN-0002-2011 Authentication of Human Cell Lines: Standardization of STR Profiling. Recommendations from the standard should be followed, including the use of at least eight core STR loci and application of match criteria (80% match threshold) to allow for a small amount of genetic drift in some cell lines.

• The GenePrint-10 System co-amplifies ten loci, including the ASN-0002 loci (TH01, TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317 and D5S818) as well as D21S11. These loci collectively provide a genetic profile with a random match probability of 1 in 2.92 × 109. This assay is only suited to human samples.

• Applications include:

  • Authentication, identification and verification of human-derived cell lines

  • Detection of co-culture contamination (the presence of multiple genetic profiles in one sample indicates contamination)

  • Sample identification and tracking, particularly in BioBanking (genotyping the same sample over time can detect sample mix-ups prior to experimentation)

  • Forensic DNA analysis (research purposes only)

  • Human identity testing (sample ID confirmation)

  • Paternity testing (research purposes only)

Human Exome ID

• A sample can be mislabelled at the point of origin or mixed up during transfers in a workflow or from one laboratory to another. It has been estimated that ~2% of samples stored in biobanks and other repositories are incorrectly annotated.

• AGRF’s Exome ID is a panel of 44 SNPs that can uniquely and cost-effectively identify a sample. The panel of SNPs has been selected to be highly polymorphic and found on a range of Illumina Exome arrays. This ensures a high likelihood of automatically generating comparison SNP data for the panel if you are performing array testing, exome or whole genome sequencing.

• Once tested, samples can be tracked through processing, ensuring sample mix-ups or incorrect annotations are detected early.

Mouse Linkage Marker Set

• The Agena Mouse Linkage Marker Set is a flexible low-density mouse panel with over 550 SNPs (spread ~3-5cM apart) across the mouse genome. The SNPs have been carefully selected to maximise the polymorphisms between common inbred strains and provide approximately 300 informative markers between common mouse crosses.

• Common applications include speed congenics and mouse strain verification.

Grape ID

• Used to identify or confirm grape varieties, this test is based on DNA profiling (fingerprinting) a sample using microsatelite markers. The result is then compared to a known database. 

Olive ID

• A genetic identification service for most commercially grown olive cultivars in Australia is commercially available through AGRF. The test is based on findings of the NOVA trial (National Olive Variety Assessment) conducted on behalf of the RIRDC, and concluded in 2005. The study found that over 50 of the most commonly grown varieties had consistent genetic makeup. Only these varieties are covered by the current test, as it was found that the other varieties tested consisted of more than one clonal variety.

Microbial ID via 16S

• All bacteria contain the 16S ribosomal RNA gene (16S), which contains conserved and variable regions. Detection of the conserved region enables all bacterial kingdoms from within a sample to be detected. Identification of the variable regions enables deeper taxonomic resolution. AGRF can screen your bacterial sample to the genus / species level via a nucleic acid based sequencing method.

Human DNA Contamination Screening

• This qualitative service detects the presence or absence of human DNA. We use a proprietary simple tandem repeat kit containing 21 human STR markers to test for the presence of human DNA in samples. While this is not a forensic-accredited identification service, it does meet the Australian Standard AS 5483-2012 in relation to testing of materials from a forensic sampling kit for DNA contamination.

We have also developed custom Genetic Identification assays for Stonefruit, Sugarcane, Rat and Mouse. If you’re interested in these or something else, please let us know by contacting your Account Manager using the link below. 

AGRF’s range of Genetic Identification services offer customised solutions for DNA typing, using sequencing and genotyping methods for a variety of identification purposes, from human cell lines and exomes through to olives, grapes and others.

We have also developed custom Genetic Identification assays for Stonefruit, Sugarcane, Rat and Mouse. If you’re interested in these or something else, please let us know by contacting your Account Manager using the link below. 

Advance your genomic research.

Your Partners in Genetic Identification.