Your Local Partners for Sanger Sequencing
With labs in Adelaide, Brisbane, Melbourne, Perth and Sydney, AGRF offers rapid and personalised Sanger Sequencing services.
We have four points of entry, depending on your specific project needs:
Full Service Sanger Sequencing
Send us your gDNA (or samples for DNA extraction) and we will PCR amplify, purify and sequence the PCR products. You can provide us with a primer design, or our bioinformatics team can design the primers for you. If you are planning a large PCR-based sequencing project, this option lets us take care of the whole process.
This service is commonly used for the identification of somatic mutations and genetic variability, such as SNPs. If you add our extraction services and custom bioinformatics analysis options, we can take you from raw tissue to analysed data.*
*Note: minimum project sizes apply.
Data Delivery
For service sequencing, once sequencing is complete you will receive the following data files for each sample submitted:
Raw trace (.ab1 file)
BLAST results against the GenBank database (.bn file)
FASTA Quality Trimmed Sequence (.fa file)
Raw, unfiltered basecall sequence (.seq file)
In addition, each batch submitted will receive a Batch Summary Report containing key quality metrics regarding the batch.
Why use AGRF’s Sanger Sequencing Service?
Easy Sample Submission
submit your samples through our online portal and then just send them through to us, or drop them off
Local Processing and Support
labs in all major capital cities
convenient drop-off locations throughout Australia, or alternatively, send your samples via Australia Post
local support from our dedicated, expert Sanger teams
Free Advice and Troubleshooting
online troubleshooting resources
on-site workshops available
advice and support just a phone call away
Quality Guarantee
AGRF Sanger Sequencing services are quality assured through NATA accreditation, to both ISO17025 (Biological Testing) and in Melbourne, to ISO15189 (Medical Testing)
Advance your genomic research.
Your Local Partners in Sanger Sequencing.