Next Generation Sequencing 

AGRF Next Generation Sequencing services are offered using Illumina NovaSeq, HiSeq and MiSeq platforms.  

Our services are run by experienced staff who are easily contactable via phone or email. We are available to give advice at any stage of your project and can assist you in finding a suitable sequencing solution. 

Our Services 

Platforms Sample preparation  Applications

Including:

  • Illumina HiSeq
  • Illumina MiSeq
  • Illumina NextSeq
  • Illumina NovaSeq           

Offering a range of options in:

Specialised applications include:

  • Expression analysis
  • Exome Sequencing
  • Diversity profiling
  • DeNovo Sequencing
  • Single-cell RNA-Seq
  • Linked-read sequencing for variant phasing and DeNovo assembly
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Whole Genome Sequencing

AGRF and the Garvan Institute's Kinghorn Centre for Clinical Genomics (KCCG) are working together to facilitate Australia's access to large whole genome sequencing through our national network. This partnership allows for genomic DNA samples to be submitted to your local AGRF node, for library preparation and Illumina HiSeq X Ten sequencing by KCCG. The whole genome sequencing service is offered on a per sample basis at 30x coverage. 
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Our Platforms

AGRF Next Generation Sequencing services are offered over multiple Illumina sequencing platforms including the NovaSeq, NextSeq, HiSeq and MiSeq.

Illumina NovaSeq: This platform is a step forward in Next Generation Sequencing, offering major advances in speed and data output.

Illumina HiSeq: This platform is the workhorse of Next Generation Sequencing. Also available in rapid mode via HiSeq 2500.

Illumina MiSeq: This platform offers a convenient package of data from small genomes and Illumina paired end sequencing.

Technical Specifications for Platforms

Sample preparation options

We offer sample preparation via a range of library kits. Each library is avaliable at up to 96 samples per lane.

DNA Sequencing


RNA Sequencing


 

10x Chromium

The 10x Genomics Chromium System dramatically improves the capabilities of existing sequencing technologies by using a microfluidics-based molecular barcoding solution that allows sequencing at much higher resolution and probes the transcriptional activity of individual cells in brand new ways.

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We also offer Library QC for client prepared libraries. This can be beneficial if your project requires a library type not currently offered as one of our services or if you want to be more experimental at this step.

Our Applications

Sequencing is available over a range of applications.  These include: 

  • Single-cell RNA-Seq
  • human genome variant phasing