Mouse Strain Genotyping

A model organism for mammalian genetic and biomedical research. 

Mouse models are a powerful tool for human genetic research due to shared physiology, rapid breeding cycles (1 mouse year = 30 human years) and over 95% similarity of the mouse genome to the human genome.  Mouse models are commonly used for studying disease and drug therapies.  Over the years several inbred mouse strains have been established and methods to distinguish the genetic differences between these strains have provided a useful tool to researchers.  At AGRF we offer two mouse genotyping methods.

Agena Mouse Linkage Marker Set

Mouse Microsatellite Genotyping

Agena Mouse Linkage Marker Set

The mouse linkage Market set (MLMS) is a panel that allows you to perform linkage mapping studies. The panel is run using our NATA accredited Agena SNP genotyping service.

The panel contains over 550 SNPs with an average distance of 3-5cM across the mouse genome and provides approximately 300 informative markers between common mouse crosses. The SNPs have been carefully selected to maximise the polymorphism between common inbred strains.

Applications:

Speed Congenics What is speed congenics?

A congenic strain is identical to a reference strain at all genetic loci except one. The differing locus is usually the transgene or knockout region of interest. Congenic strains are widely used in biomedical research because they reduce genetic variability and provide insight into the contribution of genetic background to phenotype.

Number of Informative SNPs between Common Mouse Strains


sw aj akr balb btbr c3h b6 dba fvb nod nzb
aj 430                    
akr 363 308                  
balb 377 245 322                
btbr 248 397 350 355              
c3h
403 296 341 297 390            
b6
400 451 361 396 350 400          
dba
407 405 382 389 370 269 392        
fvb 332 346 364 344 322 361 352 359      
nod 356 345 333 356 347 362 350 357 274    
nzb 328 398 348 380 327 389 345 330 340 338  
sjl 341 343 332 328 340 350 359 356 232 316  

 

1 - Unnamed

Benefit of using Mouse Linkage Marker Sets 

The MLMS can be used to preferentially select animal carrying the locus of interest for further breeding by identifying the highest percentage of recipient versus donor strain DNA. This accelerates the speed of congenic strain production. Utilizing traditional, random backcrossing methods, it would take 10 generations (upwards of 2.5 years) to produce a comparable congenic strain.

Mouse strain validation 

The panel can be used to validate in house mouse strains, ensuring consistency with established reference strains.

129S1/SvlmJ C57BLKS/J DDK/Pas MSM/Ms RIIIS/J
129S4/SvJae C57BR/cdJ FVB/NJ NOD/ShiLtJ SEA/GnJ
129X1/SvJ C57L/J  I/LnJ  NON/ShiLtJ  SJL/J 
A/J C58/J  JF1/Ms NZB/BINJ  SM/J 
AKR/J CAST/EiJ  KK/HIJ  NZW/LacJ  SPRET/EiJ 
BALB/cByJ CBA/J  LG/J  O20  ST/bJ 
BTBR_T+_tf/J CE/J  LP/J  PERA/EiJ  SWR/J 
BUB/BnJ CZECHII/EiJ  MA/MyJ  PL/J  WSB/EiJ 
C3H/HeJ  DBA/1J  MAI/Pas  PWD/PhJ   
C57BL/6J  DBA/2J  MOLF/EiJ  Qsi5   

Mouse Microsatellite Genotyping

Microsatellite Mouse Marker Set 

Over 100 microsatellite markers are available for microsatellite genotyping, these are mostly used for mouse strain validation. Select markers can be used to validate strains and parentage lines, ensuring consisteny with established reference strains. 

News

Aboriginal mitogenome analysis shows 50,000 years of connection to country

16 March 2017

Genomic analysis of hair samples collected from Australian Aboriginals across the nation have indicated that cultural connection to country has existed for as many as 50,000 years. 

The Aboriginal Heritage Project, led by the University of Adelaide’s Australian Centre for Ancient DNA recently published their findings in the journal Nature (online 8th March 2017).  AGRF is proud to be associated with the work as part of an ARC linkage grant, with the AGRF’s Operations Manager Dr John Stephen as a Partner Investigator.

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