Bioinformatics Resources

Sequencing Applications through Bioinformatics

Below is a summary of the applications that are avaliable through combining sequencing services with Bioinformatics.

 

DNA-seq

What does it offer?

Starting with genomic DNA you can sequence and assembly a genome with a reference, do de novo assembly for a genome with no reference, while exome sequencing provides an ideal method for looking for variants.

RNA-seq

What does it offer?

RNA-seq enables researchers to determine how many and which RNA transcripts are in a cell or sample; it enables identification of DNA sequences in the genome which are copied into RNA and assigned a biological function; and it enables characterization of RNA splicing.

ChIP-seq

What does it offer?

ChIP (chromatin immunoprecipitation) is used for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits, transcription factors, or other regulatory or structural proteins bound either directly or indirectly to DNA. Using high quality antibodies, protein-interacting regions of chromosomal DNA, as well as their post-translational modifications can be detected.

 

What will I receive?

The AGRF makes available data in compressed (gzipped) FASTQ format (*.fastq.gz). FASTQ format is a text-based format for storing both a biological sequence and its corresponding quality scores.  For further information on the structure of a FASTQ file see here.  Paired end sequencing generates two files, *R1.fastq.gz for mate 1, *R2.fastq.gz for mate 2.

 

Methylation
analysis

What does it offer?

Methylation analysis can be genome wide (whole genome bisulfite sequencing), targeted (Illumina HumanMethylation450 BeadChips), promoter and small region specific (Agena) or small region and CpG specific (Qiagen PyroMark)

News

Aboriginal mitogenome analysis shows 50,000 years of connection to country

16 March 2017

Genomic analysis of hair samples collected from Australian Aboriginals across the nation have indicated that cultural connection to country has existed for as many as 50,000 years. 

The Aboriginal Heritage Project, led by the University of Adelaide’s Australian Centre for Ancient DNA recently published their findings in the journal Nature (online 8th March 2017).  AGRF is proud to be associated with the work as part of an ARC linkage grant, with the AGRF’s Operations Manager Dr John Stephen as a Partner Investigator.

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